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China-Russia Workshop
November 07, 2020 |
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China-Russia Workshop
November 07, 2020 |
Prof. Xingwang Li | Huazhong Agricultural University
Abstract: High-resolution three-dimensional genome organization and its effect on transcription remain elusive in plants. Here, using an improved ChIA-PET approach, we mapped H3K4me3- and RNA polymerase II (RNAPII)-associated promoter-promoter interactions and H3K9me2-marked heterochromatin interactions at nucleotide/gene resolution in rice. The chromatin architecture was separated into different independent spatial interacting modules with distinct transcriptional potential and covered approximately 82% of the genome. Compared with inactive modules, active modules possessed the majority of active loop genes with higher density of active genes that contributed to the most of transcriptional activity in rice. In addition, promoter-promoter interacting genes tended to be transcribed cooperatively. By contrast, heterochromatin-mediated loops played structural roles in chromatin configuration and had no significant effect on gene transcription. Furthermore, we revealed the impact of genetic variation on chromatin interactions and transcription and identified a spatial correlation between the genetic regulation of eQTLs and e-traits. In summary, our strategy reveals hierarchical and modular 3D genome architecture for transcriptional regulation in rice.